Identification of Glomerella cingulata f. sp
phaseoli recombinants by RAPD markers

O.A. Camargo Junior¹, E.A. Souza¹, M.C. Mendes-Costa², J.B. Santos¹ and M.A. Soares^2
1Departamento de Biologia, Universidade Federal de Lavras (UFLA), Lavras, MG, Brasil
²Centro Universitário de Lavras, Lavras, MG, Brasil
Corresponding author: E.A. Souza
E-mail: easouza@ulfa.br

Genet. Mol. Res. 6 (3): 607-615 (2007)
Received June 4, 2007
Accepted August 10, 2007
Published September 30, 2007

ABSTRACT. We examined the capacity of strains of Glomerella cin-gulata f. sp phaseoli fungus (Colletotrichum lindemuthianum sexual stage) to form recombinants, using random amplified polymorphic DNA (RAPD). Crosses of all possible combinations between strains 40, 42, 20, 21, 22, 23, 24, 25, and 26 were made on Petri dishes using M3 culture medium. The 42 x 21 cross produced the largest number of perithecia and five asci; the respective ascospores were isolated. RAPD analysis was performed on the parents and descendants. The 62 polymorphic RAPD bands obtained were used to assess the genetic similarity using the method of Sorence and Dice and clustering analysis in the form of a dendrogram by the UPGMA method. The RAPD markers allowed identification of recombinants from the cross between strains 42 and 21 of G. cingulata f. sp phaseoli and 40 ascospores presented 63 and 49% genetic similarity with parents 2 (strain 42) and 1 (strain 21), respectively.

Key words: Colletotrichum lindemuthianum, Sexual phase, DNA marker, Recombinants, Clustering analysis