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- Splicing factors are differentially expressed in tumors
- Natanja Kirschbaum-Slager1, Graziela M.P. Lopes1, Pedro A.F. Galante1,2, Gregory J. Riggins3 and Sandro J. de Souza1
- 1Ludwig Institute for Cancer Research, São Paulo Branch, São Paulo, SP, Brazil
- 2Ph.D. Program, Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo,
- São Paulo, SP, Brazil
- 3John Hopkins University, School of Medicine, Baltimore, MD, USA
- Corresponding author: S.J. de Souza
- E-mail: [email protected]
- Genet. Mol. Res. 3 (4): 512-520 (2004)
- Received October 4, 2004
- Accepted December 16, 2004
- Published December 30, 2004
ABSTRACT. Although alternative splicing of many genes has been found associated with different stages of tumorigenesis and splicing variants have been characterized as tumor markers, it is still not known whether these examples are sporadic or whether there is a broader association between the two phenomena. In this report we evaluated, through a bioinformatics approach, the expression of splicing factors in both normal and tumor tissues. This was possible by integrating data produced by proteomics, serial analysis of gene expression (SAGE) and microarray experiments. We observed a significant shift in the expression of splicing factors in tumors in both SAGE and microarray data, resulting from a large amount of experiments. We discuss that this supports the notion of a broader association between alternative splicing and cell transformation, and that splicing factors may be involved in oncogenic pathways.
Key words: Alternative splicing, Tumorigenesis, SAGE
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