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Review
Heterologous protein production and delivery systems for Lactococcus lactis
Sébastien Nouaille1, Luciana A. Ribeiro1,2, Anderson Miyoshi2 , Daniela Pontes2,
Yves Le Loir3, Sergio Costa Oliveira2, Philippe Langella1 and Vasco Azevedo2
1Unité de Recherches Laitières et de Génétique Appliquée, Institut National de la Recherche
Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France
2Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil
3Laboratoire de Microbiologie UMR1055, Ecole Nationale Supérieure Agronomique de Rennes,
Institut National de la Recherche Agronomique, 65, rue de Saint Brieuc, CS84215,
35042 Rennes cedex, France
Corresponding author: V. Azevedo
E-mail: [email protected]
Genet. Mol. Res. 2 (1): 102-111 (2003)
Received November 27, 2002
Published March 31, 2003

ABSTRACT. Lactic acid bacteria (LAB), widely used in the food industry, are present in the intestine of most animals, including humans. The potential use of these bacteria as live vehicles for the production and delivery of heterologous proteins of vaccinal, medical or technological interest has therefore been extensively investigated. Lactococcus lactis, a LAB species, is a potential candidate for the production of biologically useful proteins. Several delivery systems have been developed to target heterologous proteins to a specific cell location (i.e., cytoplasm, cell wall or extracellular medium). A promising application of L. lactis is its use as an antigen delivery vehicle, for the development of live mucosal vaccines. The expression of heterologous proteins and antigens as well as the various delivery systems developed in L. lactis, and its use as an oral vaccine carrier are discussed.

Key words: Lactococcus lactis, Secretion, Mucosal vaccine, Antigens, Cytokines

 

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