M.R. Amorim¹, F.R. Vargas², J.C. Llerena Junior³ and M.S. Pombo-de-Oliveira^1
1Divisão de Medicina Experimental, Instituto Nacional de Câncer, Rio de Janeiro, RJ, Brasil
²Divisão de Genética, Instituto Nacional de Câncer, Unidade de Genética,
Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brasil
³Divisão de Genética, Instituto Fernandes Figueira, FIOCRUZ,
Rio de Janeiro, RJ, Brasil
Corresponding author: M.S. Pombo-de-Oliveira
E-mail: mpombo@inca.gov.br

Genet. Mol. Res. 6 (3): 500-503 (2007)
Received March 13, 2007
Accepted May 4, 2007
Published August 27, 2007

ABSTRACT. We developed a procedure for DNA extraction from small volumes of fixed cell suspensions previously prepared for conventional cytogenetic analysis. Good quality DNA was isolated with a fast and simple protocol using DNAzol reagent. This provided suitable DNA for various types of molecular analyses, including polymerase chain reaction, restriction fragment length polymorphism, denaturing high-performance liquid chromatography, and direct sequencing. This technique provides sufficient material for such test, which are important for diagnosis of neoplastic diseases in pediatric patients.

Key words: DNA extraction, DNAzol, Cell suspension, Cytogenetic